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KMID : 0380420210450040158
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Journal of Prventive Veterinary Medicine
2021 Volume.45 No. 4 p.158 ~ p.165
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Improved solid-phase competition enzyme-linked immunosorbent assay for the specific detection of antibodies against porcine parvovirus based on VP2 protein expressed in baculovirus
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Ouh In-Ohk
Lee Ju-Yeon
Moon Seo-Young
Song Jae-Young
Jang Sang-Ho
Cheong Kwang-Myun
Park Choi-Kyu
Lee Yoon-Hee
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Abstract
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A solid-phase competition enzyme-linked immunosorbent assay (ELISA), recombinant VP2 (rVP2) protein, and monoclonal antibody (mAb) were developed for the specific and sensitive detection of porcine parvovirus (PPV) antibodies in pig sera. A total of 1,544 sera samples were collected from breeding pig farms located in the Gyeongsangbuk-do Province in the Republic of Korea. The optimal operating conditions of SC-ELISA were as follows. The concentration of rVP2 proteins coated on the wells was 4 μg/mL, the swine sera were diluted 1:2, and the HRP-conjugated PPV VP2 mAb (9A8 clone) was used at 500 ng/mL. These results suggest that the SC-rVP-ELISA assay may be a valuable alternative to the current diagnostic tools used to detect PPV-specific monoclonal antibodies and broadly monitor PPV infections in domestic pigs at different breeding stages.
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KEYWORD
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porcine parvovirus (PPV), enzyme-linked immunosorbent assay (ELISA), VP2
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